Johns Hopkins University Research Technologist in Baltimore, Maryland
The Biophysics Department is looking for a bright and enthusiastic individual to join our team in studying nucleosomes and chromatin remodeling enzymes in the Bowman Laboratory as a Research Technician . This individual will be a successful candidate trained in biochemical and biophysical techniques for studying the properties and mechanisms of chromatin remodelers. We anticipate that this individual should be able to contribute significantly to ongoing projects and be considered for co-authorship.
Specific Duties & Responsibilities:
Recombinantly express and purify histones and chromatin remodeler proteins, assemble and purify nucleosomes, and perform in vitro binding and activity assays. The assays will use a range of gel- and solution-based techniques. Kinetic and binding data will be quantified and fit by nonlinear least squares.
As with most labs, some techniques (such as mutagenesis) are standard, but others (nucleosome sliding) are specialized for our projects. The Research Technician is therefore not expected to have experience with some core laboratory techniques. In the training phase, this position will be supervised in learning techniques and will assist with ongoing projects. As competency increases, they will be given more independent projects.
Must be responsible and organized, and clearly document all experiments in a laboratory journal. In addition to contributing to specific projects, this role will also help maintain laboratory equipment, keep common laboratory reagents stocked, and help maintain overall lab organization.
Minimum Qualifications (Mandatory):
- Bachelor's Degree in Biology, Chemistry or related field.
Some related post-undergraduate work experience preferred.
At least one year of wet lab experience.
Experience with protein mutagenesis, expression and purification would be a plus.
Classified Title: Research Technologist
Starting Salary Range: $15.00 - $20.00/hr (commensurate with experience)
Employee group: Full Time
Schedule: M-F, 8:30am - 5:00pm
Exempt Status: Non-Exempt
Location: Homewood Campus
Department name: Biophysics
Personnel area: School of Arts & Sciences
The successful candidate(s) for this position will be subject to a pre-employment background check.
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