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Johns Hopkins University Research Technologist in Baltimore, Maryland

The Biophysics Department is looking for a bright and enthusiastic individual to join our team in studying nucleosomes and chromatin remodeling enzymes in the Bowman Laboratory as a Research Technician . This individual will be a successful candidate trained in biochemical and biophysical techniques for studying the properties and mechanisms of chromatin remodelers. We anticipate that this individual should be able to contribute significantly to ongoing projects and be considered for co-authorship.

Specific Duties & Responsibilities:

  • Recombinantly express and purify histones and chromatin remodeler proteins, assemble and purify nucleosomes, and perform in vitro binding and activity assays. The assays will use a range of gel- and solution-based techniques. Kinetic and binding data will be quantified and fit by nonlinear least squares.

  • As with most labs, some techniques (such as mutagenesis) are standard, but others (nucleosome sliding) are specialized for our projects. The Research Technician is therefore not expected to have experience with some core laboratory techniques. In the training phase, this position will be supervised in learning techniques and will assist with ongoing projects. As competency increases, they will be given more independent projects.

  • Must be responsible and organized, and clearly document all experiments in a laboratory journal. In addition to contributing to specific projects, this role will also help maintain laboratory equipment, keep common laboratory reagents stocked, and help maintain overall lab organization.

Minimum Qualifications (Mandatory):

  • Bachelor's Degree in Biology, Chemistry or related field.

Preferred Qualifications:

  • Some related post-undergraduate work experience preferred.

  • At least one year of wet lab experience.

  • Experience with protein mutagenesis, expression and purification would be a plus.

Classified Title: Research Technologist

Role/Level/Range: ACRO37.5/02/CC

Starting Salary Range: $15.00 - $20.00/hr (commensurate with experience)

Employee group: Full Time

Schedule: M-F, 8:30am - 5:00pm

Exempt Status: Non-Exempt

Location: ​​​​​​​Homewood Campus

Department name: ​​​​​​​Biophysics

Personnel area: School of Arts & Sciences

The successful candidate(s) for this position will be subject to a pre-employment background check.

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The pre-employment physical for positions in clinical areas, laboratories, working with research subjects, or involving community contact requires documentation of immune status against Rubella (German measles), Rubeola (Measles), Mumps, Varicella (chickenpox), Hepatitis B and documentation of having received the Tdap (Tetanus, diphtheria, pertussis) vaccination. This may include documentation of having two (2) MMR vaccines; two (2) Varicella vaccines; or antibody status to these diseases from laboratory testing. Blood tests for immunities to these diseases are ordinarily included in the pre-employment physical exam except for those employees who provide results of blood tests or immunization documentation from their own health care providers. Any vaccinations required for these diseases will be given at no cost in our Occupational Health office.

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Equal Opportunity Employer:

Johns Hopkins University is an equal opportunity employer and does not discriminate on the basis of race, color, gender, religion, age, sexual orientation, national or ethnic origin, disability, marital status, veteran status, or any other occupationally irrelevant criteria. The university promotes affirmative action for minorities, women, disabled persons, and veterans.