Job Information
Johns Hopkins University Research Specialist II in Baltimore, Maryland
The Johns Hopkins University is seeking a Research Specialist II who will perform laboratory procedures, data processing, and laboratory maintenance following common or established protocols and procedures.
The Single Cell & Transcriptomics Core serves the Johns Hopkins Community as well as the world-wide scientific community. The Core provides cost effective Single Cell and Next Generation sequencing analysis enabling more researchers to access advanced transcriptomics and genomics services. The Core is a fast paced and growing lab that requires additional technicians to aid in sample processing and tracking, lab organization, primary data analysis.
Specific Duties & Responsibilities
Under the general supervision of a Core Director or Laboratory Supervisor, the Specialist is responsible for performing quality control analysis on client and Core generated RNA, DNA and library samples and returning data to clients.
Prepare libraries for and load, 10x Chromium, Mission Bio Tapestri, Next Generation sequencers or any newly acquired instrument.
Perform RNA and DNA extractions and advanced molecular biology sample processing.
Analyze raw sequencing data within Illumina BaseSpace software.
Analyze raw sequencing data for 10x methods, cell or tissue based.
Trains new hires on SOPs, operations, and maintenance of equipment in the core.
Perform general lab maintenance, including unpacking orders, signing in projects, and updating sample tracking software.
Perform routine maintenance on all instruments, such as cleaning/washing, calibration/system check, software updates, and power cycles.
Consults industry tech support to replace kits whenever something fails unexplainably and to troubleshoot what may have caused the problem and how to avoid it in the future.
Collaborate with clients/researchers to understand their desired project requirements and deadlines and to provide technical guidance on sample preparation for sequencing.
Explain any relevant methods to clients so that they can better understand the technology.
Communicate sequencing results and basic data analysis to researchers in a clear and concise manner.
Maintain and update sample submission records.
Help develop and implement standard operating procedures and best practices for the core facility.
Oversee multiple projects simultaneously, ensuring that deadlines are met, and quality standards are maintained.
Establish a collaborative learning environment within the lab, promoting continuous skill development and knowledge sharing.
Manage inventory, monitors supply levels, orders equipment, and supplies in accordance with established policies and procedures, and initiate the process for equipment repair when needed.
Teach/train students, and new technicians in the lab. Guide, direct, and facilitate problem solving for entry-level lab members on day-to-day problems.
Understand and use software packages used by SCTC for data analysis.
Adhere to all SCTC Core requirements.
Perform miscellaneous related duties as assigned.
Minimum Qualifications
Bachelor's Degree in Biology, Chemistry or related field.
Two years laboratory experience performing relevant techniques.
Master's degree, with related graduate research, may substitute for experience to the extent permitted by the JHU equivalency formula.
Preferred Qualifications
Chromium system.
Hands-on experience with 10x.
Classified Title: Research Specialist II
Role/Level/Range: ACRP/03/MB
Starting Salary Range: $40,900 - $71,600 Annually ($60,000 targeted; Commensurate with experience)
Employee group: Full Time
Schedule: M-F 8:30-5:00
Exempt Status: Exempt
Location: School of Medicine Campus
Department name: SOM Bas Sci Inst Service Centers
Personnel area: School of Medicine
Equal Opportunity Employer:
Johns Hopkins University is an equal opportunity employer and does not discriminate on the basis of race, color, gender, religion, age, sexual orientation, national or ethnic origin, disability, marital status, veteran status, or any other occupationally irrelevant criteria. The university promotes affirmative action for minorities, women, disabled persons, and veterans.