Job Information
Johns Hopkins University Research Specialist II in Baltimore, Maryland
Our laboratory develops molecular recording technologies to track mammalian development and understand how it is altered in the course of developmental disorders. We use genome engineering, high-throughput and in situ sequencing, as well as computational strategies. We have created developmental barcoding mouse models in which cells record their lineage history in their genomes. In this system, random mutations accumulate in designated synthetic loci in the genome during development, marking each lineage with a unique set of mutations - a barcode that records each cell’s lineage history in its genome. We are seeking a Research Specialist II .
Specific Duties & Responsibilities
Carries out lab projects and participates in developing assays and protocols.
May be responsible for daily research projects.
Designs experimental approaches for research projects.
Performs a range of assays and experiments, including cell culture, qPCR, gel electrophoresis, fluorescence microscopy, next-generation sequencing.
Makes significant/important scientific contributions to various aspects of research efforts and includes routine troubleshooting, independent decisions.
Plans and run set of experiments after discussion.
Processes and analyzes data into figures for scientific publications.
Manages and maintains barcoding mouse colony by ensuring the well-being of the colony and its compliance with university and governmental regulations, genotyping the mice using next-generation sequencing, analyzing the sequencing data, computing appropriate crosses based on the sequencing data to setting up the next generation of barcoding mice.
Organizes barcoding experiments. Implements timed pregnancies involving barcoded embryos and collects the embryos at the appropriate stage for further analysis.
Prepares sequencing libraries of obtained samples and performs next-generation sequencing.
Manages laboratory equipment and reagents, and orders equipment and supplies and interfaces with vendors.
Onboards new lab members, and trains students and other junior personnel in basic laboratory technique, including molecular biology, cell culture, and animals.
Assists in the preparation of grants and manuscripts and in the evaluation of funding opportunities.
Manages the social presence of the laboratory, including the lab website, twitter, and other social media accounts.
Performs miscellaneous related duties as assigned.
Minimum Qualifications
Bachelor's Degree in Biology, Chemistry or related field.
Two years laboratory experience performing relevant techniques.
Master's degree, with related graduate research, may substitute for experience to the extent permitted by the JHU equivalency formula.
Preferred Qualifications
Experience managing large and complex mouse colonies.
Experience with next-generation sequencing library preparation.
Basic knowledge of coding in MATLAB or R and Perl or Python.
Experience in mammalian cell culture technique, including culture human iPSCs.
Classified Title: Research Specialist II
Role/Level/Range: ACRP/03/MB
Starting Salary Range: $40,900 - $71,600 Annually ($60,000 targeted; Commensurate with experience)
Employee group: Full Time
Schedule: M-F/37.5
Exempt Status: Exempt
Location: School of Medicine Campus
Department name: SOM BME Molec and Cell Eng Physiology
Personnel area: School of Medicine
Equal Opportunity Employer:
Johns Hopkins University is an equal opportunity employer and does not discriminate on the basis of race, color, gender, religion, age, sexual orientation, national or ethnic origin, disability, marital status, veteran status, or any other occupationally irrelevant criteria. The university promotes affirmative action for minorities, women, disabled persons, and veterans.